1XF8
Crystal Structure of Weissella viridescens FemX (Y254F) Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979583 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.424, 101.335, 46.954 |
Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
Resolution | 15.990 - 1.900 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ne9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.990 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 28895 | |
<I/σ(I)> | 7.1 | 2.5 |
Completeness [%] | 94.9 | 97.3 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 295 | PEG 4000, sodium cacodylate, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 295K |