1XF8
Crystal Structure of Weissella viridescens FemX (Y254F) Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979583 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.424, 101.335, 46.954 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 15.990 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ne9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.990 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 28895 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 94.9 | 97.3 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | PEG 4000, sodium cacodylate, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 295K |
