1XEY
Crystal structure of the complex of Escherichia coli GADA with glutarate at 2.05 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 117.100, 117.100, 196.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.151 |
| Rwork | 0.148 |
| R-free | 0.19861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pmm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.446 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.270 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 60197 | |
| <I/σ(I)> | 9.97 | 4.46 |
| Completeness [%] | 95.6 | 91.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | PYRIDINE CHLORIDE, GLYCEROL,SODIUM CHLORIDE, SODIUM ACETATE, PEG 3000, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
