1XEY
Crystal structure of the complex of Escherichia coli GADA with glutarate at 2.05 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-6 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 117.100, 117.100, 196.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.050 |
R-factor | 0.151 |
Rwork | 0.148 |
R-free | 0.19861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pmm |
RMSD bond length | 0.018 |
RMSD bond angle | 1.446 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.270 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 60197 | |
<I/σ(I)> | 9.97 | 4.46 |
Completeness [%] | 95.6 | 91.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | PYRIDINE CHLORIDE, GLYCEROL,SODIUM CHLORIDE, SODIUM ACETATE, PEG 3000, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 290K |