1XEW
Structural biochemistry of ATP-driven dimerization and DNA stimulated activation of SMC ATPases.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97913, 0.97931, 0.97121, 0.91677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.285, 56.744, 78.869 |
| Unit cell angles | 90.00, 123.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.207 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 22600 | |
| Completeness [%] | 88.3 | 55.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 8000, glycerol, KCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
