1XEQ

Crystal tructure of RNA binding domain of influenza B virus non-structural protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2003-09-29
Wavelength(s)	0.9197
Spacegroup name	P 64 2 2
Unit cell lengths	102.032, 102.032, 108.843
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.600 - 2.100
R-factor	0.21548
Rwork	0.213
R-free	0.25567
Structure solution method	SAD
RMSD bond length	0.030
RMSD bond angle	2.211
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	2.120
High resolution limit [Å]	2.100	2.100
Number of reflections	18879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.9	278	pH 3.9, VAPOR DIFFUSION, SITTING DROP, temperature 278K