1XEL

UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	83.800, 83.800, 108.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 1.800
R-factor	0.182 *
RMSD bond length	0.014 *
RMSD bond angle	2.000 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]		1.800 *
Rmerge	0.024 *	0.137 *
Total number of observations	69535 *
Number of reflections	39398 *	6734 *
Completeness [%]	95.0 *	87 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	UDP-glucose	10 (mM)
3	1	reservoir	PEG8000	20 (%)
4	1	reservoir		600 (mM)
5	1	reservoir	CHES	50 (mM)