1XEI

THE CRYSTAL STRUCTURES OF LYSOZYME AT VERY LOW LEVELS OF HYDRATION

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-10-02
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	26.566, 56.032, 31.340
Unit cell angles	90.00, 112.20, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.188 *
Rwork	0.188
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	88% LOW HUMIDITY STRUCTURE
RMSD bond length	0.011
RMSD bond angle	0.040
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.144
High resolution limit [Å]	2.100	2.061
Rmerge	0.038	0.097
Total number of observations	7408 *
Number of reflections	4569
<I/σ(I)>	52.71	11.76
Completeness [%]	86.0	41.7
Redundancy	1.62	1.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		pH 4.6