1XEA
Crystal structure of a Gfo/Idh/MocA family oxidoreductase from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.529, 112.835, 177.214 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.650 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT and SAD |
| Starting model (for MR) | Experimental electron density map from Se-SAD phasing. |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 90383 | |
| <I/σ(I)> | 10.1 | 1.3 |
| Completeness [%] | 98.7 | 92.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 14% PEG 4000, 0.1M Tris 8.5, 5% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
