1XE7
Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly roll fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 206.800, 206.800, 206.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.750 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | YML079wp |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 81006 | |
<I/σ(I)> | 14.3 | 4 |
Completeness [%] | 98.6 | 100 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 283 | 20-24% PEG 4000, 0.1M Na-Citrate, 0.2M Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 283K |