1XE4
Crystal Structure of Weissella viridescens FemX (K36M) Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979577 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.326, 101.305, 46.331 |
| Unit cell angles | 90.00, 103.02, 90.00 |
Refinement procedure
| Resolution | 15.990 - 1.950 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ne9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.992 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 26196 | |
| <I/σ(I)> | 6.6 | 3.1 |
| Completeness [%] | 94.5 | 97.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | PEG 6000, sodium cacodylate, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 295K |
