1XE0
The structure and function of Xenopus NO38-core, a histone binding chaperone in the nucleolus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.000, 59.000, 87.200 |
| Unit cell angles | 77.00, 88.30, 60.90 |
Refinement procedure
| Resolution | 84.520 - 1.700 |
| R-factor | 0.21239 |
| Rwork | 0.208 |
| R-free | 0.25908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xb9 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.439 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.182 |
| Number of reflections | 109907 | |
| <I/σ(I)> | 9.6 | 4.2 |
| Completeness [%] | 79.7 | 88.2 |
| Redundancy | 4.5 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | PEG400, Ethylene glycol, Tris-HCl, Magnesium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
