1XDW

NAD+-dependent (R)-2-Hydroxyglutarate Dehydrogenase from Acidaminococcus fermentans

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-09-01
Detector	MARRESEARCH
Wavelength(s)	1.2545, 1.2555, 1.0500
Spacegroup name	P 61 2 2
Unit cell lengths	67.490, 67.490, 312.785
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.460 - 1.980
R-factor	0.17966
Rwork	0.177
R-free	0.22532
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	1.697
Data reduction software	DENZO
Data scaling software	XDS
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.031
High resolution limit [Å]	1.980	1.980
Number of reflections	28589
<I/σ(I)>	28.17	6.84
Completeness [%]	98.8	93.9
Redundancy	17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	15-18 % PEG MME 2000 or PEG 2000, and 0.2 M sodium or lithium acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K