1XDP

Crystal Structure of the E.coli Polyphosphate Kinase in complex with AMPPNP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	298
Detector technology	CCD
Collection date	2001-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97929, 0.97939, 0.95372
Spacegroup name	P 42 21 2
Unit cell lengths	152.000, 152.000, 150.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.337
Rwork	0.248
R-free	0.27400
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.450
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	113237
Completeness [%]	87.0	87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1,6 Hexanediol, Hepes, DTT, Magnesium chloride, AMPPNP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1,6 Hexanediol, Hepes, DTT, Magnesium chloride, AMPPNP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1,6 Hexanediol, Hepes, DTT, Magnesium chloride, AMPPNP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1,6 Hexanediol, Hepes, DTT, Magnesium chloride, AMPPNP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K