1XDA
STRUCTURE OF INSULIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.752, 78.752, 79.199 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| Rwork | 0.174 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4-IODOPHENOL INSULIN DIMER |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.278 |
| Total number of observations | 40024 * | |
| Number of reflections | 16624 | |
| Completeness [%] | 98.0 | 72.7 * |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 | HANGING DROP, 0.1M TRI-SODIUM CITRATE, 6% (W/V) TRIS, 0.02% (W/V) ZINC ACETATE, PH 8.2. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | NN304 insulin | 7.5 (mg/ml) | |
| 2 | 1 | drop | 0.02 (M) | ||
| 3 | 1 | reservoir | trisodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | Tris | 6 (%(w/v)) | |
| 5 | 1 | reservoir | zinc acetate | 0.02 (%(w/v)) | |
| 6 | 1 | reservoir | phenol solution | 5 (%(w/v)) | |
| 7 | 1 | reservoir | ethanol | 0.010-0.060ml |
