1XD7
Crystal structure of a putative DNA binding protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791, 0.9793, 0.9400, 1.100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.892, 49.362, 51.510 |
Unit cell angles | 90.00, 121.61, 90.00 |
Refinement procedure
Resolution | 37.060 - 2.300 |
R-factor | 0.26 |
Rwork | 0.260 |
R-free | 0.28500 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.055 | 0.300 |
Number of reflections | 5553 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 86.3 | 39.8 |
Redundancy | 3.5 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG 4000, Ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |