1XCK
Crystal structure of apo GroEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 1998-07-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 262.800, 283.600, 135.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.710 - 2.920 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oel |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.710 | 3.000 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 184155 | |
| <I/σ(I)> | 11.5 | 1.5 |
| Completeness [%] | 83.8 | 27.4 |
| Redundancy | 1.7 | 0.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG 4000, ammonium sulphate, hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
