1XCH
MYOGLOBIN (HORSE HEART) MUTANT WITH LEU 104 REPLACED BY ASN (L104N)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-02-17 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.566, 28.872, 35.882 |
| Unit cell angles | 90.00, 107.17, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.700 |
| R-factor | 0.179 * |
| Rwork | 0.179 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.037 |
| Data reduction software | RIGAKU |
| Data scaling software | RIGAKU |
| Refinement software | GPRLSA |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.084 |
| Number of reflections | 12442 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 83.0 |
| Redundancy | 3.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.4 | 20 MM TRIS/HCL, 1MM EDTA, PH 8.4, 8 MG/ML PROTEIN 69% AMMONIUM SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 60-62 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 68-70 (%sat) | |
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 7 | 1 | reservoir | EDTA | 1 (mM) |
