1XCD
Dimeric bovine tissue-extracted decorin, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.380, 124.110, 129.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.140 - 2.310 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 2.430 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 20012 | |
| <I/σ(I)> | 11.6 | 3.5 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 293 | PEG 400, Tris, beta-octyl-D-glucoside, sodium azide, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
