1XC8
CRYSTAL STRUCTURE COMPLEX BETWEEN THE WILD-TYPE LACTOCOCCUS LACTIS FPG (MUTM) AND A FAPY-DG CONTAINING DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92004 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.513, 92.513, 142.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.247 - 1.950 |
| R-factor | 0.17805 |
| Rwork | 0.176 |
| R-free | 0.20932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tdz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.776 |
| Data reduction software | XNEMO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.247 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 41650 | |
| <I/σ(I)> | 7.7 | 2.6 |
| Completeness [%] | 91.4 | 91.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | HEPES, SODIUM CITRATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | SODIUM CITRATE | ||
| 3 | 1 | 2 | SODIUM CITRATE |
