1XC7
Binding of beta-D-glucopyranosyl bismethoxyphosphoramidate to glycogen phosphorylase b: Kinetic and crystallographic studies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2003-09-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.87 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.733, 128.733, 116.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.360 - 1.830 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.055 | 0.479 |
Number of reflections | 84865 | |
<I/σ(I)> | 13.2 | 3.7 |
Completeness [%] | 98.3 | 97.1 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.8 | 298 | pH 6.8, SMALL TUBES, temperature 298K |