1XC6
Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 |
Unit cell lengths | 110.960, 110.960, 161.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.400 - 2.100 |
Rwork | 0.171 |
R-free | 0.18590 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.338 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.400 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.107 | 0.481 |
Number of reflections | 224036 | |
<I/σ(I)> | 12.5 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 15% PEG 8000, 50 MM Sodium Phosphate (Iodide ions were incorporated into the crystal by Quick Cryo Soaking technique at room temperature), pH 4.0, Vapor Diffusion, Hanging Drop, temperature 291K |