1XC4

Crystal structure of wild-type tryptophan synthase alpha-subunits from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-08-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54180
Spacegroup name	C 1 2 1
Unit cell lengths	162.273, 44.479, 71.519
Unit cell angles	90.00, 106.56, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.26
Rwork	0.244
R-free	0.31800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bks
RMSD bond length	0.006
RMSD bond angle	1.265
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.139	0.321
Number of reflections	12425
<I/σ(I)>	15.1
Completeness [%]	90.8	90.8
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	ammonium sulfate, trisodium citrate dihydrate, lithium sulfate monohydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K