1XC4
Crystal structure of wild-type tryptophan synthase alpha-subunits from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.273, 44.479, 71.519 |
| Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.26 |
| Rwork | 0.244 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bks |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.265 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.139 | 0.321 |
| Number of reflections | 12425 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 90.8 | 90.8 |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | ammonium sulfate, trisodium citrate dihydrate, lithium sulfate monohydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
