1XC3
Structure of a Putative Fructokinase from Bacillus subtilis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.660, 112.660, 75.087 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.350 - 2.100 |
| R-factor | 0.18145 |
| Rwork | 0.180 |
| R-free | 0.20283 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.172 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.350 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 29850 | |
| Completeness [%] | 97.0 | 98.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Ammonium sulfate, Tris, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
