1XBB

Crystal structure of the syk tyrosine kinase domain with Gleevec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Detector technology	CCD
Collection date	2003-11-10
Detector	MARRESEARCH
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	39.787, 85.706, 89.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.000 - 1.570
Rwork	0.191
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.583
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.002	1.650
High resolution limit [Å]	1.570	1.570
Rmerge	0.055	0.376
Number of reflections	43411
<I/σ(I)>	16.7	5.2
Completeness [%]	98.9	94.2
Redundancy	6.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		15% peg 3350, 100mM Tris HCl (pH 8.5)