1XBB
Crystal structure of the syk tyrosine kinase domain with Gleevec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Collection date | 2003-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.787, 85.706, 89.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 1.570 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.583 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.002 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.055 | 0.376 |
| Number of reflections | 43411 | |
| <I/σ(I)> | 16.7 | 5.2 |
| Completeness [%] | 98.9 | 94.2 |
| Redundancy | 6.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 15% peg 3350, 100mM Tris HCl (pH 8.5) |
