1XBA

Crystal structure of apo syk tyrosine kinase domain

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Detector technology	CCD
Collection date	2002-11-25
Detector	MARRESEARCH
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
格子定数 [Å]	39.785, 84.721, 90.186
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.830 - 2.000
Rwork	0.231
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.017
結合角の平均二乗偏差(RMSD) [度]	1.584
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.861	2.110
分解能 [Å] (高)	2.000	2.000
Rmerge_l_obs	0.117	0.845
独立反射数	21184
<I/σ(I)>	9.1	2
完全性 [%]	99.8	99.2
冗長性	6.6	5.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		8.5		15% peg 3350, 100mM Tris HCl (pH 8.5)