1XB9
The structure and function of Xenopus NO38-core, a histone chaperone in the nucleolus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.913, 64.549, 97.461 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 91.290 - 1.900 |
| R-factor | 0.20794 |
| Rwork | 0.204 |
| R-free | 0.25504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k5j |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.559 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.221 |
| Number of reflections | 89372 | |
| <I/σ(I)> | 14.5 | 3.24 |
| Completeness [%] | 94.0 | 94 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | PEG400, Tris-HCl, Calcium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
