1XB2
Crystal Structure of Bos taurus mitochondrial Elongation Factor Tu/Ts Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9818, 0.9824, 0.9716 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.000, 149.300, 70.000 |
| Unit cell angles | 90.00, 125.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 38985 | |
| Completeness [%] | 98.5 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 279 | 14-18% PEG 8000, 100 mM Tris-HCl pH 7.6, 200 mM Na3Citrate-2H2O, 2 mM DTT and 1 mM NaN3 , VAPOR DIFFUSION, SITTING DROP, temperature 279K |
