1XAW
crystal structure of the cytoplasmic distal C-terminal domain of occludin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.960, 109.570, 48.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.730 - 1.450 |
R-factor | 0.265 |
Rwork | 0.261 |
R-free | 0.28400 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 22041 | |
Completeness [%] | 99.2 | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 295 | amonium sulfate, pH 5.6, VAPOR DIFFUSION, temperature 295.0K |
1 | VAPOR DIFFUSION | 5.6 | 295 | amonium sulfate, pH 5.6, VAPOR DIFFUSION, temperature 295.0K |