1XAU
STRUCTURE OF THE BTLA ECTODOMAIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.54978, 2.06637 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.670, 37.542, 90.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.850 - 1.800 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.200 |
| Data reduction software | MOSFLM |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.800 | 1.830 |
| Rmerge | 0.056 | 0.185 |
| Number of reflections | 11536 | |
| <I/σ(I)> | 14.4 | 4.2 |
| Completeness [%] | 96.5 | |
| Redundancy | 3.7 | 2.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293.15 | 10mM Cadmium Chloride, 100mM Citric Acid/NaOH, 220mM Ammonium Acetate, 26% PEG 4000, 10mM Trimethylamine-HCl, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
