1XAR

Crystal Structure of a fragment of DC-SIGNR (containing the carbohydrate recognition domain and two repeats of the neck).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	82.050, 82.050, 110.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.727 - 2.250
R-factor	0.222
Rwork	0.222
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.727	2.370
High resolution limit [Å]	2.250	2.250
Number of reflections	20863
<I/σ(I)>	9.5	2
Completeness [%]	99.9	99.9
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	Protein solution: 5mg/ml protein. Reservoir solution: 3.75M Sodium formate, 0.05M Tris, pH=8.5., VAPOR DIFFUSION, HANGING DROP, temperature 294K