1XAE

Crystal structure of wild type yellow fluorescent protein zFP538 from Zoanthus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-08-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	121.160, 121.160, 111.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.206
Rwork	0.205
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xa9
RMSD bond length	0.006
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.700
High resolution limit [Å]	2.700	2.600
Rmerge	0.170
Number of reflections	29648
<I/σ(I)>	10.8	1.7
Completeness [%]	99.9	99.5
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	288	ammonium sulfate, beta-mercaptoethanol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K