1XAC

CHIMERA ISOPROPYLMALATE DEHYDROGENASE BETWEEN BACILLUS SUBTILIS (M) AND THERMUS THERMOPHILUS (T) FROM N-TERMINAL: 20% T MIDDLE 20% M RESIDUAL 60% T, MUTATED AT S82R. LOW TEMPERATURE (100K) STRUCTURE.

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-09-09
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	77.300, 77.300, 157.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.2
Rwork	0.200
RMSD bond length	0.017
RMSD bond angle	3.510 *
Data reduction software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.050
Number of reflections	22097
Completeness [%]	67.2
Redundancy	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *	100 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	10 (mM)
2	1	1	ammonium sulfate	2.2 (M)
3	1	1	glycerol	22 (%(w/v))