1XAA

3-ISOPROPYLMALATE DEHYDROGENASE, LOW TEMPERATURE (100K) STRUCTURE

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-08-08
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	77.000, 77.000, 156.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.157
Rwork	0.157
RMSD bond length	0.015
RMSD bond angle	2.990 *
Data reduction software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.058
Number of reflections	19388
Completeness [%]	59.8
Redundancy	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *	100 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	10 (mM)
2	1	1	ammonium sulfate	2.2 (M)
3	1	1	glycerol	22 (%(w/v))