1XA1
Crystal structure of the sensor domain of BlaR1 from Staphylococcus aureus in its apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.10000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.876, 104.893, 90.273 |
Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.18824 |
Rwork | 0.186 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | benzylpenicillin-acylated BlaR1 C-terminal domain PDB entry 1XA7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.296 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 95274 | |
Completeness [%] | 97.3 | 78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 291 | 18-23% PEG 3350, 0.2M NaH2PO4, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |