1XA0
Crystal Structure of MCSG Target APC35536 from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 99.8 |
| Detector technology | CCD |
| Collection date | 2004-03-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 156.236, 156.236, 68.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.21836 |
| Rwork | 0.212 |
| R-free | 0.26931 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.420 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.085 | 0.575 |
| Number of reflections | 28389 | |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 99.9 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.3 | 298 | 160mM Ammonium sulfate, pH 8.3, VAPOR DIFFUSION, temperature 298K |
