1X9Z
Crystal structure of the MutL C-terminal domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9840, 0.9793, 0.9790 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 95.992, 95.992, 140.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.610 - 2.100 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.069 | 0.481 |
Number of reflections | 36759 | |
<I/σ(I)> | 22.2 | 2.5 |
Completeness [%] | 94.5 | 97.7 |
Redundancy | 11.5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 277 | NaCl, Li2SO4, sodium acetate, pH 4.6, VAPOR DIFFUSION, temperature 277K |