1X9Z
Crystal structure of the MutL C-terminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9840, 0.9793, 0.9790 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 95.992, 95.992, 140.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.100 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.481 |
| Number of reflections | 36759 | |
| <I/σ(I)> | 22.2 | 2.5 |
| Completeness [%] | 94.5 | 97.7 |
| Redundancy | 11.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 277 | NaCl, Li2SO4, sodium acetate, pH 4.6, VAPOR DIFFUSION, temperature 277K |
