1X9T
The crystal structure of human adenovirus 2 penton base in complex with an ad2 N-terminal fibre peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 438.370, 299.825, 419.583 |
| Unit cell angles | 90.00, 103.22, 90.00 |
Refinement procedure
| Resolution | 29.980 - 3.500 |
| R-factor | 0.306 |
| Rwork | 0.306 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | electron microscopy map of ad3 dodecahedron |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.190 | 0.450 |
| Number of reflections | 354798 | |
| <I/σ(I)> | 3.5 | 1.5 |
| Completeness [%] | 54.0 | 29 |
| Redundancy | 2.1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 1.6M Ammonium sulfate, 10% dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
