1X9R
Umecyanin from Horse Raddish- Crystal Structure of the oxidised form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 65 |
| Unit cell lengths | 92.690, 92.690, 47.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.660 - 1.900 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jer |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.830 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.290 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.059 | 0.269 |
| Number of reflections | 18450 | |
| <I/σ(I)> | 45 | 16 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 8.6 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | PEG3350, Bicine, Magnesium chloride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
