1X9G
PUTATIVE MAR1 RIBONUCLEASE FROM LEISHMANIA DONOVANI
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Detector technology | CCD |
Collection date | 2004-04-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9794, 0.9793, 0.8919 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 147.438, 147.438, 147.438 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.406 - 2.410 |
R-factor | 0.22969 |
Rwork | 0.225 |
R-free | 0.27567 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.970 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.400 | 50.000 | 2.470 |
High resolution limit [Å] | 2.400 | 5.490 | 2.400 |
Rmerge | 0.075 | 0.033 | 0.918 |
Number of reflections | 10935 | ||
Completeness [%] | 98.4 | 100 | |
Redundancy | 20.8 | 19 | 20.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 290 | 0.5 ul protein 10 mg/ml, 5% DMSO, 2.5% glycerol, 250 mM NaCl, 10mM HEPES pH 7.5 0.5 ul crystallization buffer 18% PEG 1000, 50 mM MES pH 5.6, 50 mM K3PO4, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |