1X99
X-ray crystal structure of Xerocomus chrysenteron lectin XCL at 1.4 Angstroms resolution, mutated at Q46M, V54M, L58M
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9184, 0.9791 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.153, 59.153, 149.663 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.300 - 1.400 |
| R-factor | 0.17447 |
| Rwork | 0.174 |
| R-free | 0.18743 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 60727 | |
| <I/σ(I)> | 15.6 | 7.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.6 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | ammonium sulfate, MES, zinc chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
