1X8N
1.08 A Crystal Structure Of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.147, 42.775, 53.042 |
| Unit cell angles | 90.00, 94.09, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.080 |
| R-factor | 0.13411 |
| Rwork | 0.134 |
| R-free | 0.17140 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1koi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.120 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.059 | 0.270 |
| Number of reflections | 66157 | |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 98.4 | 95.1 |
| Redundancy | 5.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 300 | ammonium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
