1X8M
X-ray structure of pectin degrading enzyme 5-keto 4-deoxyuronate isomerase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.01 |
Spacegroup name | P 31 |
Unit cell lengths | 101.011, 101.011, 179.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.600 |
R-factor | 0.259 |
Rwork | 0.257 |
R-free | 0.28000 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.430 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 62299 | |
Completeness [%] | 99.1 | 91.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Sodium Citrate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Sodium Citrate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |