1X8E
Crystal structure of Pyrococcus furiosus phosphoglucose isomerase free enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.236, 73.865, 77.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.450 - 2.800 |
| R-factor | 0.2177 |
| Rwork | 0.215 |
| R-free | 0.27586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x82 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.763 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.450 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.141 | 0.439 |
| Number of reflections | 10096 | |
| <I/σ(I)> | 4.9 | 1.7 |
| Completeness [%] | 99.0 | 4.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 1.6M tri-sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
