1X7W
Crystal structure of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-23 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 145.672, 145.672, 69.236 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.730 |
R-factor | 0.14777 |
Rwork | 0.147 |
R-free | 0.18083 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ols |
RMSD bond length | 0.020 |
RMSD bond angle | 1.703 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.068 | 0.667 |
Number of reflections | 86997 | |
<I/σ(I)> | 25.2 | |
Completeness [%] | 98.9 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | PEG4000, pH 5.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K |