1X7V
Crystal structure of PA3566 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | SBC-2 |
| Wavelength(s) | 0.96498, 0.97872, 0.98024 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.555, 38.395, 63.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.760 - 1.780 |
| R-factor | 0.16715 |
| Rwork | 0.164 |
| R-free | 0.22297 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.661 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.053 | 0.378 |
| Number of reflections | 25374 | |
| <I/σ(I)> | 25.8 | 3.1 |
| Completeness [%] | 97.1 | 85.4 |
| Redundancy | 5.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 294 | Ammonium sulfate, Sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
