1X7Q
Crystal structure of HLA-A*1101 with sars nucleocapsid peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9815 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.465, 80.623, 56.570 |
| Unit cell angles | 90.00, 116.79, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.1346 |
| Rwork | 0.133 |
| R-free | 0.18070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hla |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.104 | 0.450 |
| Number of reflections | 81898 | |
| <I/σ(I)> | 15.94 | 3.14 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.84 | 3.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 25% PEG 5000 MME, 0.2M AMMONIUM SULFATE, 0.1M MES PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
