1X7O
Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793,0.9790,0.9611 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.623, 76.623, 208.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.370 |
| R-factor | 0.20766 |
| Rwork | 0.206 |
| R-free | 0.24899 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.325 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.410 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.048 | 0.248 |
| Number of reflections | 26199 | |
| <I/σ(I)> | 23.5 | 5.4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 200, MES, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
