1X79
Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5
Experimental procedure
Experimental method | MAD |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2003-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418,0.95667,0.97938,0.97952 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 155.222, 155.222, 53.052 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.810 - 2.410 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.28300 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.054 | 0.405 |
Number of reflections | 28214 | |
<I/σ(I)> | 30.1 | 2.3 |
Completeness [%] | 97.2 | 77.3 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | AMMONIUM SULFATE, TRIS, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |