1X6Z
Structure 1: cryocooled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 0.78A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.65 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 37.244, 37.244, 147.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.040 - 0.780 |
| R-factor | 0.14252 |
| Rwork | 0.142 |
| R-free | 0.15350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dzo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.986 |
| Data reduction software | d*TREK |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.040 | 0.790 |
| High resolution limit [Å] | 0.780 | 0.780 |
| Rmerge | 0.082 | |
| Number of reflections | 107451 | |
| <I/σ(I)> | 5.47 | |
| Completeness [%] | 89.8 | 41.1 |
| Redundancy | 11.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 293 | 60% Ammonium Sulfate, 0.1M Hepes, pH 8.2, VAPOR DIFFUSION, temperature 293K |
