1X6U
KDO8P synthase in it's binary complex with the product KDO8P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.934 |
Spacegroup name | I 2 3 |
Unit cell lengths | 118.530, 118.530, 118.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1phw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.680 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.307 | |
Number of reflections | 6950 | |
<I/σ(I)> | 13.1 | 5.3 |
Completeness [%] | 90.7 | 99.9 |
Redundancy | 6.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG4000, Glycerol, Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |