1X6Q
Structure 3: cryocooled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 1.51A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-10 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 37.475, 37.475, 147.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.920 - 1.510 |
| R-factor | 0.13926 |
| Rwork | 0.138 |
| R-free | 0.16050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dzo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.417 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.920 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.050 | |
| Number of reflections | 16090 | |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 91.7 | 16.3 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 293 | 60% Ammonium Sulfate, 0.1M Hepes, pH 8.2, VAPOR DIFFUSION, temperature 293K |
